Ayrin Sultana, Md Adnan Al Bachchu*, Roushan Ara, Mohammad Mosharof Hossain Bhuyain and Md Moniruzzaman1
Department of Entomology, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, 1Department of Agricultutre, Fulbari Womans College, Dinajpur, Bangladesh *Corresponding author: adnan@hstu.ac.bd
Article history: Received: 27.09.2020, Accepted: 20.12.2020, Published Online: 31.12.2020
Cite this article:
Sultana A, Bachchu MAA, Ara R, Bhuyain MMH and Moniruzzaman M. 2020. Comparative toxicity study of leaf, bark and stem extracts of eucalyptus against Sitophilus oryzae L. (Curculionidae: Coleoptera). Intl. J. Agric. Med. Plants. 1(1): 16-25.
ABSTRACT
Comparative toxicity of the extract of eucalyptus leaf, bark and stem as a botanical insecticides against rice weevil, Sitophilus oryzae L., the most common and destructive insect in all types of rice storage in Bangladesh, was evaluated based on toxicity, repellency and residual activity in the laboratory conditions (28±3°C and 70±5% RH). The extract was prepared with methanoic solvents and applied in four doses (0.5, 1.0, 1.5 and 2.0%) with control. Extracts from all three parts had toxic effects against rice weevil but the extracts of the eucalyptus stems showed the highest (mortality, 34.53%) toxic effect while extract of leaves showed the lowest (mortality, 11.20%) effect. The lower LD50 values of the stem extract also showed the highest toxic effects against rice weevils. In the residual toxicity test, the stem extract also showed the lowest number of adult emergence (36.53) and the highest percent of infestation inhibition (67.42%), but the highest number of adult emergence was recorded in the control (78.33) treatment. Among the tested plant parts of eucalyptus, the stem extract showed the highest repellent effect (73.33%) and the leaves extract was the lowest (56.33%). All levels of extract had direct toxicity, residual and repellent effects, but the highest response was obtained using 2.0% and the toxicity increased in proportion to the level of concentration. Considering the toxicity, residual and repellency effects, toxicity were found in the order of stem>bark>leaf. We therefore, suggest that the stem extract of eucalyptus are promising for environment friendly management practices against stored grain pests.
Keywords: Eucalyptus, residual toxicity, repellency effect, Sitophilus oryzae L.
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